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Figure 6 | BMC Microbiology

Figure 6

From: Suppression subtractive hybridization identifies an autotransporter adhesin gene of E. coli IMT5155 specifically associated with avian pathogenic Escherichia coli (APEC)

Figure 6

Expression of AatA in different E. coli strains. The purified fusion protein (lane 1) and total protein extract of BL21(pET32a:aatA F) (lanes 2 and 3), expressing AatAF under the control of the IPTG-inducible promoter, AAEC189(pUC18:aatA+P) expressing aatA under the control of the native promoter and AAEC189(pUC18) (lanes 4 and 5), APEC_O1 (lane 6), IMT5155 (lane7), CFT073 (lane 8) and MG1655 (lane 9) were separated on an SDS gel and blotted to polyvinylidene fluoride membrane. The membrane was then incubated with anti-AatA antibody.

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BMC Microbiology

ISSN: 1471-2180

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