Global gene expression under nitrogen starvation in Xylella fastidiosa: contribution of the σ54 regulon

BMC Microbiology

Table 2 Differentially expressed genes under nitrogen starvation in the rpoN mutant compared to the wild-type strain.

Gene ID	Product^§	Ratio (log₂)^#
Downregulated genes (positively regulated by RpoN)
XF2542*	fimbrial protein	-3.79
XF2272*	5-methyltetrahydropteroyltriglutamate homocysteine methyltransferase	-2.21
XF1819*	threonine dehydratase catabolic	-1.62
XF1121*	5,10-methylenetetrahydrofolate reductase	-1.51
XF2699	transcription termination factor Rho	-1.37
XF0180*	hypothetical protein	-1.03
XF2207	cationic amino acid transporter	-0.80
Upregulated genes (negatively regulated by RpoN)
XF1109	hypothetical protein	1.89
XF2343	recombination protein N	1.63
XF0887	mannosyltransferase	1.61
XF1830	nitrile hydratase activator	1.52
XF2551	conserved hypothetical protein	1.46
XF1658	phage-related repressor protein	1.30
XF1781	hypothetical protein	1.29
XF1117	hypothetical protein	1.24
XF2555	lysyl-tRNA synthetase	1.23
XF1469	conserved hypothetical protein	1.17
XF1078	DNA uptake protein	1.16
XF0412	nitrate ABC transporter ATP-binding protein	1.14
XF0318	NADH-ubiquinone oxidoreductase, NQO14 subunit	1.08
XF0221	hypothetical protein	0.94
XF2377	hypothetical protein	0.81

^§ Predicted function based on sequence similarity.
^# Log ratio of fluorescence intensity in strain rpoN compared to the J1a12 strain [log₂(I_rpoN/I_J1a12)], both grown up under nitrogen starvation during two hours. Microarray analyses were carried out for three independent biological samples and a gene was classified as differentially expressed if at least four of its six replicates were outside the intensity-dependent cutoff curves.
* Genes induced under nitrogen starvation in at least one point of the temporal series.

ISSN: 1471-2180