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Table 2 Strains and plasmids used in this study

From: The type IV pilin, PilA, is required for full virulence of Francisella tularensis subspecies tularensis

Strains Genotype/phenotype Source
F. tularensis   
FSC237 tularensis; SCHU S4 Human ulcer 1941, Ohio
  FSC237; ΔpilA; deletion of codons 1-135 This study
  FSC237; ΔpilC (FTT1134); in frame deletion of codons 5-405 This study
  FSC237; ΔpilQ (FTT1156); in frame deletion of codons 13-593 This study
  FSC237; ΔpilT (FTT0088); in frame deletion of codons 7-336 This study
E. coli   
Top10 F- mcrA Δ(mrr-hsdRMS-mcrBC), Φ80lacZ ΔM15 ΔlacX74 recA1 deoR araD139ara-leu)7697 galU galK rpsL (Smr) endA1 nupG Invitrogen
S17-1Λpir recA, thi, pro, hsdR-M+,<RP4:2-Tc:Mu:Km:Tn7> TpR, SmR [32]
Plasmids   
pCR®4.0 TOPO-cloning vector. AmpR, KmR Invitrogen
pDM4 Suicide plasmid. sacB; mobRP4; oriR6K; CmR [30]
pSMP22 Suicide plasmid. groESL promoter, ori T, bla, sacB [29]
pSMP50CAM 432 bp fragment of pilA including a chlorampenicol resistance cassette cloned into pSMP22. CmR This study
pAL12 2072 bp fragment of approximately 1 kb upstream and 1 kb downstream of pilC cloned in XbaI and SalI site of pDM4. CmR This study
pAL16 2122 bp fragment of approximately 1 kb upstream and 1 kb downstream of pilQ cloned in XbaI and SalI site of pDM4. CmR This study
pAL18 2133 bp fragment of approximately 1 kb upstream and 1 kb downstream of pilT cloned in XbaI and SalI site of pDM4. CmR This study