Table 2 Strains and plasmids used in this study
Strains | Genotype/phenotype | Source |
|---|---|---|
F. tularensis | ||
FSC237 | tularensis; SCHU S4 | Human ulcer 1941, Ohio |
FSC237; ΔpilA; deletion of codons 1-135 | This study | |
FSC237; ΔpilC (FTT1134); in frame deletion of codons 5-405 | This study | |
FSC237; ΔpilQ (FTT1156); in frame deletion of codons 13-593 | This study | |
FSC237; ΔpilT (FTT0088); in frame deletion of codons 7-336 | This study | |
E. coli | ||
Top10 | F- mcrA Δ(mrr-hsdRMS-mcrBC), Φ80lacZ ΔM15 ΔlacX74 recA1 deoR araD139 (Δara-leu)7697 galU galK rpsL (Smr) endA1 nupG | Invitrogen |
S17-1Λpir | recA, thi, pro, hsdR-M+,<RP4:2-Tc:Mu:Km:Tn7> TpR, SmR | [32] |
Plasmids | ||
pCR®4.0 | TOPO-cloning vector. AmpR, KmR | Invitrogen |
pDM4 | Suicide plasmid. sacB; mobRP4; oriR6K; CmR | [30] |
pSMP22 | Suicide plasmid. groESL promoter, ori T, bla, sacB | [29] |
pSMP50CAM | 432 bp fragment of pilA including a chlorampenicol resistance cassette cloned into pSMP22. CmR | This study |
pAL12 | 2072 bp fragment of approximately 1 kb upstream and 1 kb downstream of pilC cloned in XbaI and SalI site of pDM4. CmR | This study |
pAL16 | 2122 bp fragment of approximately 1 kb upstream and 1 kb downstream of pilQ cloned in XbaI and SalI site of pDM4. CmR | This study |
pAL18 | 2133 bp fragment of approximately 1 kb upstream and 1 kb downstream of pilT cloned in XbaI and SalI site of pDM4. CmR | This study |