Skip to main content

Table 1 Bacterial strains and plasmids used in the study.

From: Characterization and functional analysis of seven flagellin genes in Rhizobium leguminosarum bv. viciae. Characterization of R. leguminosarum flagellins

Strains and Plasmids Relevant characteristics Source or Reference
Escherichia coli strains   
DH5α endA1, hsdR17, supE44, thi-1, recA1, gyrA96, relA1,(argF-lacZYA), U169, φ 80dlacZ ΔM15 Invitrogen
S17.1 Spr. RP4 tra region, mobilizer strain [69]
Rhizobium leguminosarum strains   
3841 biovar viciae, JB300 derivative, Smr [70]
VF39SM biovar viciae, Smr [71]
VF39SM flaA- VF39SM flaA-, Smr,Nmr This work
VF39SMflaA+ VF39SMflaA- complemented with flaA, Smr, Nmr,Gmr This work
3841 flaA- gusA-Nm cassette insertion in 3841 flaA, Smr, Nmr This work
3841flaA+ 3841flaA- complemented with flaA, Smr, Nmr,Gmr This work
VF39SM flaB- Spectinomycin cassette insertion in VF39SM flaB, Smr,Spr This work
3841 flaB- Spectinomycin cassette insertion in 3841 flaB, Smr, Spr This work
VF39SM flaC- gusA-Nm cassette insertion in VF39SM flaC, Smr, Nmr This work
3841 flaC- gusA-Nm cassette insertion in 3841 flaC, Smr, Nmr This work
VF39SM flaD- gusA-Nm cassette insertion in VF39SM flaD, Smr, Nmr This work
3841 flaD- gusA-Nm cassette insertion in 3841 flaD, Smr, Nmr This work
VF39SM flaE- gusA-Nm cassette insertion in VF39SM flaE, Smr, Nmr This work
3841 flaE- gusA-Nm cassette insertion in 3841 flaE, Smr, Nmr This work
VF39SM flaH- Neomycin-resistance cassette insertion in VF39SM flaH, Smr, Nmr This work
3841 flaH- Neomycin-resistance cassette insertion in 3841 flaH, Smr, Nmr This work
VF39SM flaG- Tetracycline-resistance cassette insertion in VF39SM flaG, Smr, Tcr This work
3841 flaG- Tetracycline-resistance cassette insertion in 3841 flaG, Smr, Tcr This work
3841 flaA/B/C/D- 3841 strain with mutations in flaA/B/C/D, Smr, Nmr This work
VF39SM flaA/B/C/D- VF39SM strain with mutations in flaB/C/D, Smr, Nmr This work
VF39SM flaB/C/D- VF39SM flaA/B/C/D - complemented with flaA; Smr, Nmr, Gmr This work
3841 flaB/C/D - 3841 flaA/B/C/D - complemented with flaA; Smr, Nmr , Gmr This work
Plasmids   
pCR2.1-TOPO Cloning vector, Ampr , Kmr Invitrogen
pJQ200SK Suicide vector with sacB system; Gmr [32]
pJQ200mp18 Suicide vector with sacB system; Gmr [32]
pCRS530 Contains a promoterless gusA-Nm cassette [33]
pBSL99 Contains kanamycin-resistance cassette [36]
pBSIISK+ Cloning vector, Ampr Stratagene
pBS::flaD 3'-Km-flaA 5' flaA 5' fragment (from pCR2.1::flaA 5') subcloned into pBS::flaD 3'-Km, Ampr , Kmr This work
pJQmp18:: flaD 3'-Km-flaA 5' flaD 3'-Km-flaA 5' fragment subcloned from pBS::flaD 3'-Km-flaA 5' into pJQmp18, Gmr , Kmr This work
pHP45Ω Contains omega-spectinomycin cassette; Spr [34]
pHP45:ΩTc Contains tetracycline-resistance cassette; Tcr [35]
pBBR1-MCS5 Broad-host-range cloning vector, Gmr [72]
pFus1 pMP220 derivative with promoterless gusA, Tcr [33]
pBBR1-MCS5:3841flaA Broad-host-range cloning vector containing flaA gene (with promoter region) from 3841 This work
pBBR1-MCS5:VF39SMflaA Broad-host-range cloning vector containing flaA gene (with promoter region) from VF39SM This work
pFus1::flaB flaB promoter introduced into pFus1, Tcr This work
  1. AprAmpicillin resistance, GmrGentamicin resistance, NmrNeomycin resistance, SmrStreptomycin resistance, SprSpectinomycin resistance, TcrTetracycline resistance