Map of plasmid pRKaraRed. Some restriction sites are shown. tet A is the tetracycline resistance gene for plasmid selection in E. coli and in P. aeruginosa. oriT is a region for plasmid transfer in P. aeruginosa. Expression of lambda Red genes (gam, bet and exo) driven by P
promoter are regulated by repressor AraC. The nucleotide sequence of pRKaraRed was deposited in GenBank under the accession number GU186864.