Figure 4From: Damage of Streptococcus mutans biofilms by carolacton, a secondary metabolite from the myxobacterium Sorangium cellulosumEffect of carolacton concentration on the membrane damage of S. mutans biofilms. Biofilms were grown for 24 h under anaerobic conditions and stained using the LIVE/DEAD BacLight Bacterial Viability kit. Green and red fluorescence was determined, and biofilm damage was calculated as reduction of the fluorescence ratio green/red compared to untreated controls. Each data point is the average of triplicate samples. Standard deviations are given for data points determined in at least three independent experiments.Back to article page