Analysis of fusion sequence in fragment NA2. (A) Location of chromosomal deletion ends and fusion junction. Left and right deletion termini were characterized by stepwise PCR mapping. Deleted and fused regions are indicated by dashed and shaded lines, respectively. Kp, Kpn I. (B) Southern analysis of fusion fragment with probe N2, which was prepared using primers 236 and 239. (C) Junction sequence, showing no obvious homology between the original sequences.