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Table 2 Bacterial strains and plasmids used in this work

From: The T box regulatory element controlling expression of the class I lysyl-tRNA synthetase of Bacillus cereus strain 14579 is functional and can be partially induced by reduced charging of asparaginyl-tRNAAsn

Strains, plasmids Relevant characteristics Reference or source
Strains   
E. coli   
TG-1 supE hsd Δ5 thi Δ(lac-proAB) F'[traD 36] [24]
TP611 recBC hsdR - M - cyab10pcn [25]
B. cereus 14579 wild type isolate [34]
B. subtilis   
168 trpC2 Laboratory stock
1A717 amyE::erm EmR [32]
1A765 trpC2 lys [35]
NF33 trpC2 lys amyE::pBCJ307 (PlysK (T box)lacZ) CmR This study
NF52 trpC2 amyE::pNF48 (PlysK (T box)lysK) SpecR This study
NF54 trpC2 amyE::pNF48 (PlysK (T box)lysK) SpecR DlysS KanR This study
NF58 trpC2 amyE::pBCJ307 (PlysK (T box)lacZ) CmR asnS::pNF40 (Pspac asnS) EmR This study
NF60 trpC2 amyE::pBCJ307 (PlysK (T box)lacZ) CmR asnS::pNF40 (Pspac asnS) EmRpMap65 (P pe -lacI) PhR This study
NF113 trpC2 lysS::pNF112 (PlysK (T box)lysS) CmR This study
NF204 trpC2 amyE:: pBCJ307 (PlysK (T box)lacZ) EmR CmR This study
NF205 trpC2 lysS::pNF112 (PlysK (T box)lysS) CmR amyE:: pBCJ307(PlysK (T box)lacZ) EmR This study
NF206 trpC2 amyE::pNF48 (PlysK (T box)lysK) SpecR DlysS: KanR amyE::pBCJ307(PlysK (T box)lacZ)CmR This study
BCJ363 trpC2 amyE::pBCJ307 (PlysK (T box)lacZ) CmR This study
BCJ366 trpC2 amyE::pBCJ307 (PlysK (T box)lacZ) CmR lys::pXZ2 (Pspac lysS) EmR This study
BCJ367 trpC2 amyE::pBCJ307 (PlysK (T box)lacZ) CmR lys::pXZ2 (Pspac-lysS) EmR pMap65 PhlR This study
Plasmids   
pBluescript2 KS(-) cloning vector ApR Stratagene, La Jolla CA
pMutin4 integration vector for B. subtilis [27]
pBCJ102 pBluescript based vector containing transcription terminator cassettes ApR [29]
pBCJ144 vector to replace part of B. subtilis lysS with KanR [29]
pBCJ307 vector with transcriptional fusion of B. cereus lysK promoter and T box with lacZ CmR This study
pDG268 vector to generate lacZ promoter fusions at the amyE locus by double crossover ApR CmR [26]
pDG1730 vector for integration at the amyE locus in B. subtilis SpecR [30]
pXZ2 Vector to placing B. subtilis lysS under control of IPTG inducible Pspac promoter EmR This study
pMUTIN-XZ pMUTIN4 with the lacZ gene removed EmR This study
pMap65 replicating B. subtilis plasmid encoding penP-lacI PhlR KanR [28]
pNF30 plasmid with B. cereus lysK promoter and T box element in pBCJ102 ApR This study
pNF40 B. subtilis asnS promoter on 516 bp fragment in pMUTIN-XZ This study
pNF48 B. cereus lysK promoter and lysK gene cloned into pDG1730 This study
pNF112 the lysK promoter and T box element (423 bp) fused to B. subtilis lysS (672 bp fragment) This study