Figure 5

Acanthamoeba invades the locust suboesophageal ganglion and occasionally present in the lumen of the locust foregut. (i) (A) Suboesophageal ganglia of control locust (×100); (B) Suboesophageal ganglia of infected locust (×100); (C) Suboesophageal ganglia of control locust at higher magnification (×400); (D) Suboesophageal ganglia of infected locust at higher magnification (×400). Note that the trophozoites of amoeba (indicated by arrowheads in D) were detected in the suboesophageal ganglion of infected locusts, but not in control locusts (C). Histopathological damage in suboesophageal ganglia from infected locusts (B) was also evident, when compared with the ganglia in control locusts (A). The above images are representative micrographs of the genotype T4, but, similar results were observed with the T1 genotype. (ii) (A) Foregut of control locust (×250); (B) Foregut of infected locust (×250); (C) Foregut of infected locust at higher magnification (×400). Trophozoites of amoeba (indicated by arrow in C) were identified in the lumen of the foregut of some of the locusts. No damage to the wall of the foregut was observed in the infected locusts. Note that the above images are representative micrographs of the genotype T4, but, similar results were observed with the T1 genotype.