Figure 2
Cell viability and growth under the ethanol stress. Cell viability of ethanol- and inhibitor-tolerant mutant Saccharomyces cerevisiae NRRL Y-50316 (●) and its parental inhibitor-tolerant strain NRRL Y-50049 (○) in response to 8% (v/v) ethanol challenge as measured by OD600 on a liquid YM of 2% glucose (A) and culture appearance of cell growth on a solid YM of 2% glucose (B). The time point at the addition of ethanol to the medium was designated as 0 h. Cell growth on YM plate was evaluated 7 days after incubation at 30°C.