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Table 1 Quantitative real time PCR and corresponding microarray data of selected genes.

From: Brucella melitensis VjbR and C12-HSL regulons: contributions of the N-dodecanoyl homoserine lactone signaling molecule and LuxR homologue VjbR to gene expression

BME

Loci

Gene Function

Condition (growth phase)

Change (Fold)

   

qRT-PCR

Microarray

I 0984

ABC-Type β-(1,2) Glucan Transporter

ΔvjbR/wt (ES)

-2.5

-2.1

II 0151

Flagellar M-Ring Protein, FliF

ΔvjbR/wt (ES)

-7.9

-2.2

II 1069

Adhesin, AidA

ΔvjbR/wt (SP)

-1.9

-1.5

I 0561

Membrane-Bound Lytic Murein Transglycosylase B

ΔvjbR/wt (SP)

-1.7

-2.0

II 0025

Attachment Mediating Protein VirB1

ΔvjbR/wt (SP)

-4.1

-2.6

I 0831

UDP-3-O-[3-hydroxymyristoyl] Glucosamine N-Acyltransferase

wt + AHL/wt (ES)

2.2

2.3

II 0151

Flagellar M-Ring Protein, FliF

wt + AHL/wt (ES)

-3.8

-2.1

II 0838

Succinoglycan Biosynthesis Transport Protein, ExoT

wt + AHL/wt (ES)

-1.7

-4.3

II 1116

LuxR Family Transcriptional Regulator, VjbR

wt + AHL/wt (SP)

-2.9

-

I 1758

LuxR Family Transcriptional Regulator, BlxR

wt + AHL/wt (SP)

27.5

-

I 0155

Putative Allantoin Permease

wt + AHL/wt (SP)

-1.7

-1.4

II 0025

Attachment Mediating Protein VirB1

wt + AHL/wt (SP)

-2.5

-2.2

II 0753

ABC-Type Sorbitol/Mannitol Transport Inner Membrane Protein

ΔvjbR/ΔvjbR + AHL (ES)

1.5

2.5

I 1758

LuxR Family Transcriptional Regulator, BabR

ΔvjbR/ΔvjbR+AHL (SP)

99.5

-

  1. A (-) indicates genes excluded for technical reasons or had a fold change of less than 1.5. qRT-PCR values were calculated by the ΔΔCt method normalized to 16s rRNA and are relative to the wildtype, averaged from 3 independently isolated samples, performed in triplicate in a minimum of three assays. ES, Exponential growth phase; SP, Stationary growth phase.