Development of a versatile tool for the simultaneous differential detection of Pseudomonas savastanoi pathovars by End Point and Real-Time PCR

Table 2 Nucleotide sequences of PCR primers and probes used and developed in this study.

Primer/Probe^a	Sequence (5'-3')	Position^b	Product size (bp)	Accession Number
Psv F	GGCGATGTTCTCAGCGGATTTG	24	388	FM253081
Psv R	GATCAAGTGTCCAAGGAAGTGAAGG			FM253082
Psv RT-F	CGGATTTGGTTTGCGGGGTA	38	298	FM253083
Psv RT-R	AATGGGGTGACACTAAAAATTGTGAA			FM253084
Psv RT-P	(HEX)CTCGTGCGATCTAAACAGCCGTAGC(BHQ-1) ^c	278		FM253085
Psn F	ACCCCTCATTGTAACGGATG	1	349	AM051225
Psn R	TCCCCGGAATTCAACACTTA			AM051226
Psn RT-F	GCTCATTCGCTTGTTATCACTTCA	181	169	AM086621
Psn RT-R	TCCCCGGAATTCAACACTTA			AM051226
Psn RT-P	(FAM)TACGCCCGACGCCCGAGCCA(BHQ-1) ^c	206		FM253086
Psf F	CGCCTGCTGTACTCCTCGG	1	412	AM055834
Psf R	TCGACCTGTCTAAGGCCC			AM055835
Psf RT-F	CAGCTCATCCATTAATAGGGCAAG	207	227	AM086622
Psf RT-R	GGGCAGTGTCAGGGGATG			FM253088
Psf RT-P	(Texas Red)CTTGTACCGAAGCGTGCCGTCTGC(BHQ-2) ^c	237		FM253087

^a F, forward; R, reverse; RT, RealTime; P, probe.
^b Starting nucleotide position of forward primers and TaqMan^® probes on target sequences.
^c BHQ-1 and BHQ-2 are quencher molecules available from the manufacturer.

ISSN: 1471-2180