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Table 2 Comparison of M9 medium and defined artificial seawater medium (DASW) with respect to four main components tested

From: Natural transformation of Vibrio cholerae as a tool - Optimizing the procedure

Component

M9 medium*

Enriched M9 medium (this study)

DASW#

NaCl

9 mM

259 mM

234 mM

HEPES

0 mM

50 mM

50 mM

MgSO4

2 mM

32 mM

27.5 mM

CaCl2

0.1 mM

5.1 mM

4.95 mM

  1. * Sigma; supplemented as recommended by the manufacturer
  2. # As published [8, 9]