Figure 5
From: Transcriptome analysis of the mobile genome ICEclc in Pseudomonas knackmussii B13
Carbon substrate-dependent transcript linkage in the region at the outermost ICE clc left end. A) Gene organization, reverse-transcribed regions and PCR amplicons. Arrows to the left point to inferred promoters. B) RT-PCR results for amplicon (a). C) idem for amplicon (b). D) Amplicon (c). E) Amplicon (d). All RNAs sampled from cultures during stationary phase after growth on the indicated carbon source. Glc, glucose. Frc, fructose. 3-CBA, 3-chlorobenzoate. Numbers below point to independent replicate reactions. -, PCR but without RT-step.+, PCR on B13 genomic DNA as template.