Figure 2

Reverse transcriptase-PCR amplifications of the analyzed transcript connections indicated in Figure 1. Numbers above amplicons indicate the examined region in ICEclc numbering; numbers below the calculated amplicon size. 'Minuses' are negative control reactions with PCR only without reverse-transcriptase step to verify DNA contamination. Different panels are reactions run on the same gel but not necessarily in consecutive lanes. Electronic images were auto-leveled and relevant lanes were placed side-by-side using Adobe Photoshop CS3. Std, DNA size standard (in kilobase-pairs, kb). At least one negative control was performed on every batch of purified RNA.