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Figure 4 | BMC Microbiology

Figure 4

From: Characterization of hemin-binding protein 35 (HBP35) in Porphyromonas gingivalis: its cellular distribution, thioredoxin activity and role in heme utilization

Figure 4

Hemin binding of various rHBP35 proteins. Two μg each of rHBP35(Q22-P344) (lane 1), rHBP35 (Q22-P344 with C48S C51S) (lane 2), truncated rHBP35(M135-P344) (lane 3), or lactoferrin as a negative control (lane 4) was treated with or without 1.5 μl of 1.25 mM hemin for 2 h at room temperature. A, CBB staining; B, peroxidase activity staining. Arrowheads indicate the hemin binding proteins.

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