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Table 2 Sequences and fluorescent dyes for primers and hybridization probes used for real-time PCR.

From: Acetic acid increases the phage-encoded enterotoxin A expression in Staphylococcus aureus

Target Primer/probe Nucleotide sequence (5' → 3')
sea ESA-1 ACG ATC AAT TTT TAC AGC
  ToxA reverse CCG AAG GTT CTG TAG AAG T
  ToxA-Fluo1 CCT TTG GAA ACG GTT AAA ACG AAT AAG AAA-FL1
  ToxA-Red1 LC-R640-TGT AAC TGT TCA GGA GTT GGA TCT TCA-p2
rrn rRNA forward TGT CGT GAG ATG TTG GG
  rRNA reverse ACT AGC GAT TCC AGC TT
  Probe 1 GGA CAA TAC AAA GGG CAG CG-FL
  Probe 2 LC-R705-ACC GCG AGG TCA AGC A-p3
  1. 1The donor probe is labeled with fluorescein (FL) at the 3" end.
  2. 2The acceptor probe is labeled with LC Red640 (LC-R640) at the 5" end and the 3" hydroxy group is phosphorylated (p).
  3. 3The acceptor probe is labeled with LC Red705 (LC-R705) at the 5" end and the 3" hydroxy group is phosphorylated (p).