Skip to main content

Table 1 Primer used for conventional PCR and results from PCR analysis of four S. aureus strains.

From: Acetic acid increases the phage-encoded enterotoxin A expression in Staphylococcus aureus

Primer name1 Nucleotide sequence (5' → 3') Primer location2 Annealing temperature (°C) PCR results
     Mu50 MW2 Newman SA45
a forward TAT TCA TTG CCC TAA CGT T 789421 49 + + - +
a reverse CCG TCT AGC CAT AAA TTG ATC 789842      
b forward TAT TCA TTG CCC TAA CGT G 783956 51 - - + -
b reverse CCG TCT AGC CAT AAA TTG ATT 784377      
c forward GGC AAG ATG GTT ATC ATG 789043 47 + + - +
c reverse CGA TTA TTA TCA TGT AAC G 789799      
d forward GTT CTG ATG AGA ACT ATG 781925 48 - - + -
d reverse CGT CTC CGC AAT TTT C 782948      
e forward GGC TAT AGA TGG ATT AC 793236 47 + + - +
e reverse AGA GCT TCG TCA ATT TCA 794180      
f forward GGT AGA CAA GGC AGG TAA TAG 787832 55 - - + -
f reverse GTG GAC TTC CTA CAA CGC 788235      
g forward CAT TGA ATG GTT AGT TGT AC 761697 50 - + - +
g reverse GTC CAA GTT ATA CAT TAT CGG 762676      
h forward GAA CGC GTC TAT AGA AAA G 782755 51 + - - -
h reverse GTC CAA GTT ATA CAT TAT CGG 783832      
  1. (+) amplification occurred in PCR using the primer pair and genomic DNA from the S. aureus strain listed. (-) no PCR amplification was observed.
  2. 1Primer names indicate the physical position of PCR amplicon in Figure 6.
  3. 2Primer location indicates the position of the first 5'-nucleotide within the annotated genomes.