Table 1 Primers used for PCR amplification of ORF2--ORF5 and NSP2 from PRRSV
From: Phylogenetic analysis and molecular characteristics of seven variant Chinese field isolates of PRRSV
Genes | Primer sequence |
|---|---|
ORF2 | 5'-ACGAAGCTTATGAAATGGGGTCTATG-3' |
| Â | 5'-TATCTCGAGTCACCGTGAGTTCAAAAG-3' |
ORF3 | 5'-TTCATGATTTTCAGCAATGGCTAA-3' |
| Â | 5'-GATGGTGATGTACACGGGCGT-3' |
ORF4 | 5'-ACGGCGGCAATTGGTTTCACCTA-3' |
| Â | 5'-CGTGGTCAAGCATTTCCCCAACATA-3' |
ORF5 | 5'-CCTGAGACCATGAGGTGGGG-3' |
| Â | 5'-TTTAGGGCATATATCATCACTGG-3' |
NSP2 | 5'-TGAYTGGRATGTTGTGCTYCCTGG-3' |
| Â | 5'-ATGCGAGARAGCCAYTCCTGCGT-3' |