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Table 3 Relative quantificationa of the expression of GS (glnA1), NADP-GDH (msmeg_5442) and L_180 NAD-GDH (msmeg_4699) when M. smegmatis was exposed to prolonged periods of nitrogen limitation.

From: Glutamate dehydrogenase and glutamine synthetase are regulated in response to nitrogen availability in Myocbacterium smegmatis

Time (hours)

Gene

 
 

glnA1

P-value

MSMEG_4699

P-value

MSMEG_5442

P-value

0.5

2 ± 0.5

0.001

0.5 ± 0.1

0.001

0.5 ± 0.1

0.001

1

3 ± 0.6

0.001

0.6 ± 0.05

0.001

0.5 ± 0.08

0.001

2

67 ± 38

0.001

13 ± 4

0.001

2 ± 0.9

0.901

4

58 ± 43

0.001

18 ± 15

0.001

3 ± 3

0.272

  1. a The relative change in gene expression when M. smegmatis was exposed to nitrogen starvation was compared to gene expression after M. smegmatis exposed to 60 mM (NH4)2SO4 for 1 hours (time point zero). SigA was used as the internal reference gene. Values >1 reflect an up-regulation of gene expression whereas values <1 represent a down-regulation of expression in relation to the non-regulated internal reference, sigA.
  2. * statistically significant gene regulation (p < 0.05)
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BMC Microbiology

ISSN: 1471-2180

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