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Table 2 Glutamine synthetase specific activities determined by the γ-glutamyl transferase assay when M. smegmatis was exposed to conditions of nitrogen limitation (3 mM (NH4)2SO4) and nitrogen excess (60 mM (NH4)2SO4).

From: Glutamate dehydrogenase and glutamine synthetase are regulated in response to nitrogen availability in Myocbacterium smegmatis

(NH4)2SO4 Concentration (mM)

Time (hours)

Specific activity (U)

p-value*

3 mM

0

45 ± 17

 
 

0.5

57 ± 12

0.01

 

1

63 ± 12

0.27

 

2

78 ± 16

0.00

 

4

103 ± 17

0.00

60 mM

0

76 ± 2

 
 

0.5

50 ± 1

0.00

 

1

47 ± 5

0.08

  1. * The p-values given show the statistical significance of the change in GS specific activity between time points. p < 0.05 (in bold) was regarded as a statistically significant change in specific activity from the previous time point.