Figure 6

Nuclear extracts obtained from L. amazonensis promastigotes contain LaTRF bind activity. Electrophoretic mobility shift assays (EMSA) were done using radiolabeled double-stranded telomeric DNA (LaTEL) as probe. Protein:DNA complexes were separated in a 4% PAGE in 1X TBE. In lanes 2-6, EMSA was done with nuclear extracts obtained from L. amazonensis promastigotes. In lane 2, the reaction was done in the absence of competitors. In lanes 3 and 4, binding reactions were done respectively, in presence of 100 fold excess of double-stranded non-specific DNA (poly [dI-dC] [dI-dC]) and 20 fold excess of non-labeled LaTEL. In lane 5, a supershift assay was done with anti-LaTRF serum and in the presence of 20 fold excess of non-labeled LaTEL and in lane 6, the supershift assay shown in lane 5 was done in the absence of competitors.