SDS-PAGE analysis of the extracted M. tuberculosis H37Rv proteins. Panel A, shows the whole cell lysate of M. tuberculosis H37Rv, the aqueous phase proteins and the lipid phase proteins after Triton X-114 extraction. The fractions for LC-MS/MS analysis of the lipid phase is indicated. Explanation of the fraction numbers: (1) >160 kDa, (2) 105-160 kDa, (3) 75-105 kDa, (4) 50-75 kDa, (5) 35-50 kDa, (6) 30-35 kDa, (7) 25-30 kDa, (8) 15-25 kDa, (9) 15-10 kDa, (10) <10 kDa. Panel B shows western blot analysis of the aqueous and lipid phases using a polyclonal rabbit antiserum against a BCG cell wall fraction. The molecular weight standards are shown on the left hand side of each panel.