Propionibacterium acnes infection induces upregulation of inflammatory genes and cytokine secretion in prostate epithelial cells

Table 2 List of genes that are downregulated upon P. acnes infection.

Gene name	Description	Fold upregulation
FOS	V-fos FBJ murine osteosarcoma viral oncogene homolog	-3
HMGB1	High-mobility group box 1	-3
TLR4	Toll-like receptor 4	-4
UBE2V1	Ubiquitin-conjugating enzyme E2 variant 1	-3

Semiconfluent RWPE-1 monocell-layers were infected with P. acnes at a MOI of 16:1. After 24 h infection, the cells were harvested, mRNA was collected and cDNA was prepared. The cDNA corresponding to 84 inflammation-associated genes was quantified with qRT-PCR and compared with cDNA prepared from non-infected cells. Inclusion criteria: > 2-fold down-regulation, (p = 0.05).

ISSN: 1471-2180