Figure 6
From: Cell-associated hemolysis activity in the clinical strain of Pseudomonas fluorescens MFN1032
Construction of MFN1030 hrpU operon disrupted mutant. phrp U indicates the promoter of hrpU operon. tet is the tetracycline resistance gene of pME3087. The length of each hrc gene is indicated below its designation (in base pairs). 3'hrc R indicates hrc R gene deleted in 5'-end and 5'hrc T indicates hrc T gene deleted in 3'-end. The arrow above the genes represents the operon transcription. A bold line represents DNA from pME3087. Hind III and Eco RI are enzymes used to clone hrc RST in pME3087. Unknown indicates putatives hrc genes located upstream or downstream hrc RST genes.