Validation of the genes observed as more or less induced in the A. fumigatus ΔAfcrzA mutant. Fold increase in mRNA levels after the incubation with 200 mM CaCl2 for 10 and 30 minutes of (A) AfrcnA (Afu2g13060), (B) AfrfeF (Afu4g10200), (C) Af BAR adaptor protein (Afu3g14230), (D) Af AAA ATPase (Afu4g04800), and (E) AfScf1 (Afu1g17370). Real-time RT-PCR was the method used to quantify the mRNA. The measured quantity of the mRNA in each of the treated samples was normalized using the CT values obtained for the β-tubulin (Afu1g10910) mRNA amplifications run in the same plate. The relative quantitation of all the genes and tubulin gene expression was determined by a standard curve (i.e., CT -values plotted against logarithm of the DNA copy number). The results are the means ± standard deviation of four sets of experiments. The values represent the number of times the genes are expressed compared to the corresponding control strain grown before adding 200 mM CaCl2 (represented absolutely as 1.00).