General overview of the process for maintenance, queries, and analysis of gene sequences using the PseudoMLSA Database server http://www.uib.es/microbiologiaBD/Welcome.html. The isolate, strain or Pseudomonas species information can be easily queried by searching against several fields. Furthermore, users can do sequence-based searches against database including user's own sequence datasets. Advanced searches are possible via configurable BLAST parameters. A more fine-tuned clustering analysis can be carried out with programs included in the PHYLIP package.