Figure 3

Effect of prothioconazole + fluoxastrobin (a), prothioconazole (b) and azoxystrobin (c) on extracellular H ₂ O ₂ concentrations. Conidia at a concentration of 10⁶ conidia/ml were challenged with a tenfold dilution series of fluoxastrobin + prothioconazole, azoxystrobin and prothioconazole starting from 0.5 g/l + 0.5 g/l, 0.83 g/l and 0.67 g/l. H₂O₂ was measured at 4 h (solid line), 24 h (dashed line) and 48 h (point dashed line) using TMB (trimethylbenzidine) as a substrate in the presence of an overdose of peroxidase. The H₂O₂ concentrations were calculated based on a standard curve included in each experiment. Each data point is the result of three repetitions and the experiments were repeated twice in time. Different letters at each data point indicate differences from the control treatment at 4 h (**), 24 h (*) and 48 h after analysis with a Kruskall-Wallis and Mann-Whitney test with a sequential Bonferroni correction for multiple comparisons.