Effect of prothioconazole + fluoxastrobin (a), prothioconazole (b) and azoxystrobin (c) on extracellular H
concentrations. Conidia at a concentration of 106 conidia/ml were challenged with a tenfold dilution series of fluoxastrobin + prothioconazole, azoxystrobin and prothioconazole starting from 0.5 g/l + 0.5 g/l, 0.83 g/l and 0.67 g/l. H2O2 was measured at 4 h (solid line), 24 h (dashed line) and 48 h (point dashed line) using TMB (trimethylbenzidine) as a substrate in the presence of an overdose of peroxidase. The H2O2 concentrations were calculated based on a standard curve included in each experiment. Each data point is the result of three repetitions and the experiments were repeated twice in time. Different letters at each data point indicate differences from the control treatment at 4 h (**), 24 h (*) and 48 h after analysis with a Kruskall-Wallis and Mann-Whitney test with a sequential Bonferroni correction for multiple comparisons.