Table 1 Oligonucleotides
RT PCR | Gene set | |
|---|---|---|
RT_BAB1_1374_Bam HI.F | GGATCC ACACGCGATTTCCTTTCATC | 1 |
RT_ureA2 _Bam HI.R | GGATCC CATCACCTCTTCGACGGTTT | 1, 2 |
RT_BAB1_1375.F | AAGGTCCTGCCAGTACAACG | 2 |
RT_ureA 2.F | AAACCGTCGAAGAGGTGATG | 3 |
RT_ureC 2.R | CGCAGATCCTTCTCGATTTC | 3 |
RT_ureC 2.F | ACAGTCGATCTCGCTCAACC | 4 |
RT_BAB1_1381.R | CTTGATAAGGATTGGCACGA | 4 |
RT_BAB1_1381.F | ACCTGATCCGTGAAAACGTC | 5 |
RT_BAB1_1383.R | GAAAGAACAGTCCCGTCAGC | 5 |
RT_BAB1_1383.F | GGATACAACCAAGCCTGCAT | 6 |
RT_BAB1_1386.R | GGCATTGCGGATGATAAGTT | 6 |
RT_BAB1_1386.F | GCTTTTTCTCTGGGCCAAAT | 7 |
RT_BAB1_1388.R | GACAGGGAAAGCTTGTCGAG | 7 |
ΔureT | ||
U_BMEI0642_Xba I.F | TCTAGA GACCCAGACCATAACGCTTG | |
U_BMEI0642_Bam HI.R | GGATCC CTGCCATGGAGGCCTCCT | |
BMEI0642.F | AGGAGGCCTCCATGGCAGGGATCC CCTGAGCCTGATTTCTGGA | |
D_BMEI0642_Pst I.R | CTGCAG GACCGATCCGTCATTGACAT | |
aphT | ||
aphT.F | ATACTGCAG ATTAGAAAAACTCATCG | |
aphT.R | TCACACAGGAAACAGCTATG | |
ΔnikO | ||
BAB1_1388 XbaI.R | ACGTTCTAGA CAATATCTGCGTGCTCTCCA | |
RT_BAB1_1388.R | GACAGGGAAAGCTTGTCGAG | |
BAB1_1388 Bgl II.F | CTCGACAAGCTTTCCCTGTCAGATCT CCACCTGCATTATGTCGAG | |
BAB1_1388 Pst I.R | ACGTCTGCAG CATTATCGATAGCGGCCTTG |