Table 3 Strains and plasmids used in this study.
Strains or plasmids | Relevant characteristics | Reference or source |
|---|---|---|
Strains | ||
H. pylori | ||
CCUG17874 | wild-type strain | CCUG, Sweden |
hp0256 KO | CCUG17874 Δhp0256::Cmr | This study |
P1 | wild-type strain | [57] |
P79 | P1 Strr | [58] |
P79-hp0256 KO | P79 Δhp0256::Cmr | This study |
P79-0256/pIR203K04 | P79 Δhp0256::Cmr with pIR203K04 (Kanr) | This study |
P79-0256/pIR0601 | P79 Δhp0256::Cmr with pIR0610 (Kanr) | This study |
S. typhimurium | ||
SJW1103 | wild-type strain | [59] |
MKM40 | SJW1103 ΔfliJ | [59] |
MKM40-pQE60 | SJW1103 ΔfliJ with empty pQE-60 | This study |
MKM40-pQE60-0256 | SJW1103 ΔfliJ with pQE-60-0256 | This study |
E. coli | ||
One Shot TOP10 | F- mcr A Δ(mrr-hsd RMS-mcr BC) ф80lac Z ΔM15 Δlac X74 rec A1 ara Δ139 Δ(ara-leu)7697 gal U gal K rps L (Strr) end A1 nup G | Invitrogen, USA |
Plasmids | ||
pIR203K04 | kanamycin resistance cassette (Kanr) | [51] |
pIR0601 | pIR203K04 with hp0256 gene under the control of hp0601 promoter | This study |
C-term His-tagged expression vector (Ampr) | ||
pQE-60 | pQE-60 with hp0256 gene | Qiagen, Germany |
pQE-60-0256 | This study |