Bioluminescence enables detection of thoracic and extra thoracic signals in cortisone acetate treated mice. (A): Time response study of luminescence emission from mice immunosuppressed either with cortisone acetate (A, B) or with a combination of cortisone acetate and clodrolip (C-E). Mice were intranasally infected with 2 × 106 conidia. A cohort of 10 mice received liposomes as a control prior to infection (F). Images of day one (D1) and two (D2) post-infection are shown. Luminescence was monitored 10 min after intraperitoneal injection of D-luciferin. Images from ventral (V) and dorsal (D) views of the sinus region, six days after infection (D6) of mice treated with both, cortisone acetate and clodrolip, are shown (E). The graph in (G) represents the average of the total photon flux measured from a defined thoracic region from each individual animal of the respective cohort. (H): Time course of total luminescence from chest, abdomen and head regions from animals receiving the combination of cortisone acetate and clodrolip.