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Figure 3 | BMC Microbiology

Figure 3

From: Phenotypic silencing of cytoplasmic genes using sequence-specific double-stranded short interfering RNA and its application in the reverse genetics of wild type negative-strand RNA viruses

Figure 3

Ablation of RSV F by anti-F dsRNA. Anti-F dsRNA, RSV infection, and immunostaining of A549 monolayer were performed as described under Materials and Methods. Right panel shows the nuclear staining of the same cells using DAPI (Blue). Note the substantial reduction of F (Green) with as low as 3 nM anti-F dsRNA, and reduction to background levels by 20 nM dsRNA.

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