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Figure 1 | BMC Microbiology

Figure 1

From: Characterization of Norwalk virus GI specific monoclonal antibodies generated against Escherichia coli expressed capsid protein and the reactivity of two broadly reactive monoclonal antibodies generated against GII capsid towards GI recombinant fragments

Figure 1

Schematic diagram of rNV96-908 capsid fragments expressed in E. coli. The solid bar represents the full length of the rNV96-908 capsid protein. Numbers besides the bar show nucleotide numbers of NV96-908 capsid. Numbers above the bar indicate the planned position of the fragment edges not the actual positions. Small open boxes with numbers indicate each fragment. The N-terminal fragment consists of fragments 1–7 and the C-terminal fragment consists of fragments 8–17. These fragments were constructed using restriction enzyme sites in primers for ligation convenience (Table 2).

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