Fig. 3
From: The gut microbiota facilitate their host tolerance to extreme temperatures
Long-term extreme temperature stress changes the gut microbiota composition. (a) Principal component analysis (PCA) based on weighted UniFrac analysis of ASVs. Each symbol represents a single sample of rectal after 60 days of RT, Cold and Heat. n = 4. (b) Comparison of phylum-level proportional abundance of the intestinal microbiota of up to 60 days RT, Cold and Heat mice. (c) Hierarchical clustering diagram using the average-neighbor (HC-AN) method comparing rectal of 60 days RT, Cold and Heat mice. Associated heatmap shows the relative abundance of representative ASVs selected for p < 0.05, obtained with a Welch t test comparison of the two groups and then grouped into families. The P value for the correlation between temperature and gut microbiota was corrected using a false discovery rate. One representative ASV with the greatest difference between the two group means from each family is selected for inclusion in the heatmap diagram. ASVs are shown as: Phylum and Genus. (d) Phylum level proportional abundance in rectal. n = 4. All values show mean ± SEM. Significance was calculated using non-paired two-tailed Student’s t test. *p ≤ 0.05, **p ≤ 0.01