Fig. 6

YC128 showed significant stronger in vitro anti-phagocytosis ability than that of HAP-AB strain. Peritoneal macrophages isolated from 5 mice and each mouse macrophages were distributed into 6 wells. Then, 20 μl per well of YC156 and YC128 bacterial suspension were added into the cell culture. After two hours later, the cell culture medium was collected to quantify the extracellular bacterial number. Then the wells were washed using ice-cold PBS and sterile water added into wells to lysis macrophage. Two hours later, the suspension was cultured to quantify the intracellular bacterial number. a Intracellular bacterial number. b Extracellular bacterial number