Fig. 2

Phylogenetic tree of D-Ala-D-(X) ligases. Phylogenetic tree of ligases, those highlighted in blue are present among Enterococcus. The following accessions were used to construct the tree VanA [Enterococcus faecium] (AAA65956.1), VanD [Enterococcus faecium] (AAM09849.1), VanM [Enterococcus faecium] (ACL82961.1), VanC2 [Enterococcus casseliflavus] (AAA60990.1), VanE [Enterococcus faecalis] (ABA71731.1), VanL [Enterococcus faecalis] (ABX54687.1), VanN [Enterococcus faecium] (AEP40500.1), VanF [Paenibacillus popilliae] (WP_006285587.1), D-alanine–D-serine ligase VanG [Clostridioides difficile] (WP_021362548.1), D-alanine–D-serine ligase VanG [Clostridioides difficile] (WP_021425673.1), VanG [Enterococcus faecalis] (AAQ16273.1), D-alanine–D-alanine ligase [Enterococcus faecalis] (WP_002379157.1), D-alanine–D-alanine ligase [Enterococcus faecium] (WP_002293424.1), D-alanyl-alanine synthetase A [Staphylococcus aureus subsp. aureus str. JKD6008] (ADL66141.1), D-alanine–D-alanine ligase [Leuconostoc mesenteroides subsp. mesenteroides J18] (AET29676.1), D-ala D-ala ligase [Lactiplantibacillus plantarum subsp. plantarum ATCC 14917] (EFK27904.1), VanP [Roseburia sp. 499] (WP_075721811.1), VanP [Enterococcus faecium] (WP_222893641.1), VanI [Desulfitobacterium dichloroeliminans] (WP_041219811.1), VanO [Rhodococcus] (WP_209928075.1). The sequences were aligned using muscle [41] and the tree was constructed using RAxML-NG v1.2.0 [42] with 200 bootstrap replicates