Fig. 8From: Characterization of the major autolysin (AtlC) of Staphylococcus carnosusZymograms of S. carnosus TMW 2.146 and TMW 2.2515 and their respective atlC mutants. Samples were taken from the supernatant of the respective cultures over 7 days. These samples were sterile-filtered and subsequently denatured by Laemmli loading dye. The denatured proteins were separated by size in a zymogram. Later, the proteins were renatured using a renaturation buffer (50 mM Tris -HCl pH 8 with 0.5% Triton X-100). Lytic proteins cleave Micrococcus lysodeikticus cells in the gel and form bright bands in the otherwise gray gel. First, many bands are formed in the wild-type strains, and no bands are visible in the knockout mutants. Furthermore, one can see that the banding pattern of the wild-type strains changes over the 7 daysBack to article page