Fig. 2
From: Characterization of the major autolysin (AtlC) of Staphylococcus carnosus
Zymogram of S. carnosus TMW 2.146 and TMW 2.2515 wild-type (WT) and the atlC mutant. Whole-cell hydrolysates are separated in zymograms according to their size, and then proteins are refolded in renaturation buffer (50 mM Tris -HCl pH 8 with 0.5% Triton X-100). The active lytic enzymes cleave the Micrococcus lysodeikticus cells in the gel, resulting in bright bands. The molecular sizes of the lytic bands can be determined by the size marker. In the WT, eight bands (B1 - B8) can be identified. No bands can be found in the knockout mutant