Fig. 5

Human-derived bacteria modulated gut microbiota associated with gut barrier. (a) Heatmap of 303 ASV abundances in mice according to LEfSe analysis. Blue represented ASVs highest in the healthy control, black represented ASVs highest in the mice with gut dysfunction, and green represented ASVs highest in the mice with good gut barrier under the help of the bacterial strains. (b) The relative abundance of the 13 genus/species changed by the isolated bacteria. In the box plot, the bottom and top were the 25th and 75th percentile, respectively. A line within the box marked the median. Whiskers above and below the box indicated the maximum and minimum values. Differences were assessed by Mann-Whitney test. (c) Spearman rank correlation heatmap of gut barrier parameters and PCoA coordinates/ 13 significantly different taxa in colonic content samples. Colors red and blue denoted positive and negative association, respectively. The intensity of the colors represented the degree of associations assessed by the Spearmen’s correlations. The black dots in the blue/red cells indicated the associations were significant (p < 0.05). ^&P < 0.1, *P < 0.05, **P < 0.01, ***P < 0.001 vs. DSS + PBS group by ANOVA followed by LSD post hoc test. n = 3–5 mice per group for the 16S rRNA gene sequencing of colonic content bacteria. LR: Lactobacillus rhamnosus, LP: Lactobacillus plantarum, PA: Pediococcus acidilactici, EF: Enterococcus faecium, EC: Escherichia coli