Meat and meat products as potential sources of emerging MDR Bacillus cereus: groEL gene sequencing, toxigenic and antimicrobial resistance

Table 1 Illustrates the oligonucleotide sequences used in PCR assay and their specific amplicons

	Primers	Sequences	Amplicons (bp)	References
Confirmatory gene Chaperonin protein	groEL	F:TGCAACTGTATTAGCACAAGC T R:TACCACGAAGTTTGTTCACTACT	533	[25]
hbl complex	hblA	F: AAGCAATGGAATACAATGGG R: AGAATCTAAATCATGCCACTGC	1154	[33]
	hblB	F: AAGCAATGGAATACAATGGG R: AATATGTCCCAGTACACCCG	2684
	hblC	F:GATACTCAATGTGGCAACTGC R:TTGAGACTGCTCGTCTAGTTG	740
	hblD	F:ACCGGTAACACTATTCATGC R:GAGTCCATATGCTTAGATGC	829
nhe complex	nheA	F:GTTAGGATCACAATCACCGC R:ACGAATGTAATTTGAGTCGC	755
	nheB	F:TTTAGTAGTGGATCTGTACGC R:TTAATGTTCGTTAATCCTGC	743
	nheC	F: TGGATTCCAAGATGTAACG R: ATTACGACTTCTGCTTGTGC	683
Enterotoxin	cytK	F: ACA GAT ATC GGI CAA AAT GC R: CAA GTI ACT TGA CCI GTT GC	421	[34]
Emetic toxin	ces	F:GGTGACACATTATCATATAAGGTG R:GTAAGCGAACCTGTCTGTAACAACA	1271	[34]
Phospholipase C	pc-plc	F:GAGTTAGAGAACGGTATTTATGCTGC R: CTACTGCCGCTCCATGAATCC	411	[35]
Resistance genes	bla1	F: CATTGCAAGTTGAAGCGAAA R:TGTCCCGTAACTTCCAGCTC	680	[36]
	bla2	F: TTGTCGATTCTTCTTGGGATG R: CCCCTACTTCTCCATGACCA	483	[36]
	tetA	F: GGCGGTCTTCTTCATCATGC R: CGGCAGGCAGAGCAAGTAGA	502	[37]
	tetB	F:CATTAATAGGCGCATCGCTG R:TGAAGGTCATCGATAGCAGG	930	[37]
	ermA	F: TCTAAAAAGCATGTAAAAGAA R:CTTCGATAGTTTATTAATATTAGT	645	[38]

ISSN: 1471-2180