Fig. 4

BCG-induced trained immunity in non-adherent THP-1 cells. a Schematic of short-term trained immunity. b The concentration of IL-6 secreted by trained THP-1 cells after LPS restimulation. c The apoptosis measured by flow cytometry 24 h after LPS stimulation. d The cell viability of BCG-trained THP-1 cells before and 24 h after LPS stimulation. Pre-stim: before LPS stimulation; post-stim: 24 h post LPS stimulation. e The CFUs of C. albicans (3 h post infection) after infecting BCG-trained THP-1 cells. ns P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001; # P < 0.0001. Data shown are the mean ± SD, representative of at least twice repeated experiments