Model 1: Active Inflammatory state ( inflammatory state induced 18 h before the bacterial stimulation) | |
K18h | bacteria in co-culture with CaCo2/HT29 (control) |
18 h | bacteria in co-culture with CaCo2/HT29 in inflammatory state induced 18 h prior bacteria addition |
Model 2: Protection against the inflammatory state (inflammatory state induced 3 h after bacterial stimulation) | |
K3h: | bacteria in co-culture with CaCo2/HT29 (control) |
3 h: | bacteria in co-culture with CaCo2/HT29 in inflammatory state induced 3 h after bacteria addition |
Baseline and shared controls | |
Baseline | bacteria in MRS medium under anaerobic conditions (conditions recommended for Bifidobacterium culture) |
RPMI | bacteria in RPMI 1640 medium |
RPMI + TNF-α | bacteria in RPMI 1640 medium with the addition of an inflammatory factor |
MRS medium | bacteria in MRS medium under aerobic conditions |