Metagenomic and culture-dependent approaches unveil active microbial community and novel functional genes involved in arsenic mobilization and detoxification in groundwater

Table 1 Primer sequences used for the detection of bacterial 16S rRNA gene, arsenite resistance gene, oxidizing gene, and corresponding annealing temperature used for PCR

Target gene	Primers	Sequences (5ʹ → 3ʹ)	Amplicon size (bp)	Annealing T (^oC)	Reference
16S rRNA	27F	5′- AGAGTTTGATCCTGGCTCAG-3′	~ 1400- 1450	55	Hoque et al., 2020 [24, 27]
16S rRNA	1492R	5′- GGTTACCTTGTTACGACTT-3′	~ 1400- 1450	55	Hoque et al., 2020 [24, 27]
*aio*A	aoxBM1-2F	5’CACTTCTGCATCGTGGGNTGYGGNTA-3′	1100	52	Quemeneur et al., 2008 [15]
*aio*A	aoxBM3-2R	5′- TGTCGTTGCCCCAGATGADNCCYTTYTC-3′	1100	52	Quemeneur et al., 2008 [15]
*ars*B	darsB1F	5′- GGTGTGGAACATCGTCTGGAAYGCNAC-3′	750	55	Achour et al., 2007 [29]
*ars*B	darsB1R	5ʹ-CAGGCCGTACACCACCAGRTACATNCC-3ʹ	750	55	Achour et al., 2007 [29]

ISSN: 1471-2180