Table 1 Bacterial strains and plasmids used in this study
From: Contribution of pilus type 2b to invasive disease caused by a Streptococcus agalactiae ST-17 strain
Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
E. coli | ||
 DH5α | F- φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk-, mk+) phoA supE44 λ- thi-1 gyrA96 relA1 | Invitrogen |
 Mach1™-T1R | F- φ80(lacZ)ΔM15 ΔlacX74 hsdR(rK-mK+) ΔrecA1398 endA1 tonA | Invitrogen |
S. agalactiae | ||
 COH1 | GBS wild type | Rosini et al. |
  Δbp-1 | COH1 knockout (KO) deleted of the gene coding for the backbone protein of pilus 1 (BP-1 or GBS80) | Rosini et al. |
  Δbp-2b | COH1 knockout (KO) deleted of the gene coding for the backbone protein of pilus 2b (BP-2b) | This study |
  Δbp-2b+ | Δbp-2b complemented with pAM401_ bp-2b | This study |
Plasmids | ||
 pJRS233 | 6.0 kb; ColE1 ori; temperature-sensitive E. coli-streptococcal shuttle vector | Perez-Casal et al. |
 pJRS233_ bp-2b | pJRS233-derived containing overlapping flanking sequences of bp-2b gene | This study |
 pAM401/gbs80P + T | 11.5 kb; Cmr; ColE1 ori; E. Coli-streptococcal shuttle vector pAM401 containing promoter and terminator of gbs80 gene | Rosini et al. |
 pAM401_ bp-2b | pAM401/gbs80P + T-derived containing entire bp-2b coding sequence | This study |