Table 1 Quality of results

qPCR

r²

0.993

0.939

0.996

efficiency

78.3%

79.8%

104%

Whole genome sequencing of spiking organisms

Genomic coverage of assembly

40.2×

113.8×

N/A

N50

221,548

177,674

N/A

Number of contigs

281

67

N/A

MiSeq sequencing

Cluster density (clusters/mm²)

991

1198

1326

Data quality after trimming

Number of reads (billions)

2.96–5.57

4.27–8.47

2.74–6.20

Minimum read length

50

50

50

Maximum read length

251

251

251

Average read length

217–229

193–232

172–206

Data set size after down sampling

Number of reads (billions)

2.96

4.27

2.74