Table 1 Methods routinely used in the two centers and additional assay used to confirm FilmArray GI results
From: FilmArray™ GI panel performance for the diagnosis of acute gastroenteritis or hemorragic diarrhea
Pathogen | Methods | |||
|---|---|---|---|---|
Laboratory Aa | Laboratory Bb | |||
In use | Analysis of discrepant results | In use | Analysis of discrepant results | |
Clostridium difficile | immunochromatographic test | Xpert® C. difficile/Epi (real-time PCR) | Allplex™ GI assay | Xpert® C. difficile/Epi (real-time PCR) |
Plesiomonas shigelloides | none | none | Allplex™ GI assay | none |
Salmonella spp | direct plating - culture | BD MAX™ Enteric Bacterial Panel | Allplex™ GI assay | BD MAX™ Enteric Bacterial Panel |
Yersinia enterocolitica | direct plating - culture | none | Allplex™ GI assay | none |
Campylobacter spp. (jejuni, coli and upsaliensis) | direct plating - culture | BD MAX™ Enteric Bacterial Panel | Allplex™ GI assay | BD MAX™ Enteric Bacterial Panel |
Vibrio spp. and V. cholerae | direct plating - culture | none | Allplex™ GI assay | none |
Enteroaggregative E. coli (EAEC) | direct plating - culture | none | Allplex™ GI assay | none |
Enteropathogenic E. coli (EPEC) | direct plating - culture | none | Allplex™ GI assay | none |
Enterotoxigenic E. coli (ETEC) | direct plating - culture | none | Allplex™ GI assay | none |
E.coli O157 | agglutination test | none | Allplex™ GI assay | none |
Shiga-like toxin producing E.coli (STEC) | direct plating culture/immunochromatographic test | BD MAX™ Enteric Bacterial Panel | Allplex™ GI assay | BD MAX™ Enteric Bacterial Panel |
Shigella/Enteroinvasive E. coli (EIEC) | direct plating - culture | BD MAX™ Enteric Bacterial Panel | Allplex™ GI assay | BD MAX™ Enteric Bacterial Panel |
Cryptosporidium | direct microscopy/ immunochromatographic test | Allplex™ GI assay | direct microscopy/immunochromatographic test | Allplex™ GI assay |
Entameba histolytica | direct microscopy/ immunochromatographic test | Allplex™ GI assay | direct microscopy/ immunochromatographic test | Allplex™ GI assay |
Cyclospora cayetanensis | direct microscopy/ immunochromatographic test | Allplex™ GI assay | direct microscopy/ immunochromatographic test | Allplex™ GI assay |
Giardia lamblia | direct microscopy/ immunochromatographic test | Allplex™ GI assay | direct microscopy/ immunochromatographic test | Allplex™ GI assay |
Adenovirus, | real-time PCR (all AdV strains) | PCR/sequencing | FTD | PCR/sequencing |
Rotavirus A,B | immunochromatographic test | real-time RT-PCR | FTD | Allplex™ GI assay |
Astrovirus, | immunochromatographic test | real-time RT-PCR | FTD | Allplex™ GI assay |
Sapovirus, | real-time RT-PCR | Allplex™ GI assay | FTD | Allplex™ GI assay |
Norovirus | real-time RT-PCR | Allplex™ GI assay | FTD | Allplex™ GI assay |