Fig. 3
From: DFI-seq identification of environment-specific gene expression in uropathogenic Escherichia coli
RT-qPCR verification of differentially expressed genes identified in the DFI-seq experiment. RT-qPCR was performed on bacterial RNA isolated from UTI89 grown in human urine and LB, respectively. a and b Expression ratios (log2 scale) of genes differentially expressed in human urine compared to LB, with P < 0.05. c Gene expression ratios (log2 scale) of uniformly expressed genes (P > 0.05). A pairwise fixed reallocation randomisation test was performed [45] and exact p-values can be found in Additional file 11: Table S2