Strains or plasmids | Relative characteristics | Source or reference |
---|---|---|
Strains | ||
E. coli DH5α | F−, Ф80d lacZ∆M15, ∆(lacZYA-argF)U169, endA1, recA1, hsdR17(rk−, mk+) deoR, thi-1, supE44, λ−, gyrA96(Nalr), relA1 | Invitrogen |
E. coli SM10(λ pir) | thi thr leu tonA lacY supE recA::RP4-2-TC::Mu Km r λpir | [53] |
B. cenocepacia AU1054 | Clinical isolate | BCCM-LMBP |
AU1054∆copR | AU1054 derivative with copR deletion | This study |
AU1054∆lipA | AU1054 derivative with lipA deletion | This study |
Plasmids | ||
PCRII-TOPO | Cloning vector; ori lacZ Km+ | Invitrogen |
pRK2013 | ori colE1, RK2 derivative, KanR, mob +, tra + | ATCC |
pGPI-SceI | ori R6K,TpR, mob +, carries I-SceI cut site | [37] |
pGPI∆copR | pGPI-SceI carrying 5’- and 3’-flanking regions of copR for mutagenesis of copR | This study |
pGPI∆lipA | pGPI-SceI carrying 5’- and 3’-flanking regions of lipA for mutagenesis of lipA | This study |
pDAI-SceI | ori pBBR1,TetR, mob +, P dhfr , FLAG epitope, carries I-SceI cut site | [37] |
pDA-copR | pDAI-SceI with ORF of copR replacement of SceI gene | This study |
pDA-lipA | pDAI-SceI with ORF of lipA replacement of SceI gene | This study |