Fig. 5

Hfqs Expressed in Other Bacterial Species. a Appearance of His-Hfq3 mutants, constitutively expressed from the pQE80 vector in E. coli strain YN585, on MacConkey-Ampicillin plates incubated for ≈ 16 h at 37 °C. His-Hfq1 and His-Hfq3-expressing cells are provided for reference. Numbers utilized here and in the text are the B. anthracis residue numbers. b β-galactosidase assay on MacConkey plate scrapings of YN585 strains expressing His-Hfq3 mutants ≈ 16 h at 37 °C. Bars reflect the average of measurements on three independent cultures, each obtained from an independent transformant; error bars depict +/- standard error of the mean (SEM). β-galactosidase values for reference E. coli strains cultured on MacConkey agar (Δhfq rpoS-lacZ MG1655; hfq+ rpoS-lacZ MG1655) were 54.4 ± 0.7 and 205 ± 11 Miller Units, respectively. c Comparison of growth phenotypes of Hfq2, Hfq3, Hfq3-R27A, and GFP overexpression in B. anthracis and B. subtilis; plates depict LB incubation at 37 °C in air (left) and NBY/bicarbonate incubation at 37 °C in 15% CO₂ (right)