Fig. 3
From: Detailed analysis of c-di-GMP mediated regulation of csgD expression in Salmonella typhimurium
Complementation of the rdar morphotype and csgD expression phenotypes of GGDEF/EAL mutants of S. typhimurium UMR1. Complementation by the GGDEF protein STM4551 (a) and the EAL protein STM3611 (b) in respective mutants. Catalytically inactive proteins did not restore csgD expression and the rdar morphotype. c Rdar morphotype formation and CsgD levels of S. typhimurium UMR1 upon the chromosomal replacement of the EAL to AAL motif of EAL protein STM4264. Cells were grown for 24 h at 28°C on LB without salt agar plates supplemented with ampicillin (100 μg ml−1) and 0.1% L-arabinose